Sodium and potassium content determination in Gatorade Sports Drink by Flame Photometry
Chemijos laboratorinis darbas. Sodium and potassium content determination in Gatorade Sports Drink by Flame Photometry. + sample emission values. Standard concentrations. + emission readings. Standard concentrations Na + emission reading. Na + sample emission values. Reference List.
A sports drink is used in association with sport or exercise- it is consumed either before exercise, during the exercises or recovering after physical activities (Sports Dietitians 2009). To make the final consumed product, variety of nutrients and other substances will be dissolved in water. The aims of sports drink is to stimulate rapid fluid absorption, to supply carbohydrate as a substrate for use during exercise, to speed rehydration, to reduce the physiological stress of exercise, and to promote recovery after exercise activities (Sports Dietitians 2009). The traditional ingredients in sports drink without the flavours and colouring are water, carbohydrates, sodium (Na) and potassium (K).
A traditional and simple method for sodium and potassium determination in biological samples or fluids is emission Flame Photometry. It is used extensively for the alkaline and alkali earth metals (group A1 and group A2 in the periodic table). This method relies on principle that an alkali metal drown into non-luminous flame will ionise, absorb energy from the flame and then emit light at a particular wavelength characteristic. The intensity of emission is proportional to the concentration of the element in the solution (Practical 2 n.d.). The emitted light is converted into a voltage which is detected by photocell. Because Na+ and K+ emits light of different wavelength (color), Na is bright orange and K gives a purple color, their emission can be measured by using an appropriate color filters (Practical 2 n.d.)
The purpose of this experiment was to determine sodium and potassium levels in Gatorade Sports drink. A calibration curve was set up for K and Na, using emission values form the standards and prepared samples. The calibration curve from the standards then was used to determine accurate concentrations of sodium and potassium in the samples.
1000ppm (mg.l-1) stock standard solution was given of sodium and potassium, from which the working standard solutions were prepared.